Based on the modified ramp and fire technique, a novel injection seeding approach with real-time resonance tracking is successfully demonstrated in a single-frequency Nd:YAG pulsed laser. Appling a high-frequency sinusoidal modulation voltage to one piezo actuator and an adjustable DC voltage to another piezo actuator for active feedback, single-mode laser output with high-frequency stability is obtained, and the effect of the piezo hysteresis on the frequency stability can be eliminated for a laser diode pumped Q-switched Nd:YAG laser at a repetition rate of 400 Hz.